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Cusabio

SKU:CSB-PA884504LA01HU

SLC28A3 Antibody

SLC28A3 Antibody

Regular price $166.00 USD
Regular price Sale price $166.00 USD
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Size
Release Date 2022-04-01
Product Code CSB-PA884504LA01HU
Short Name SLC28A3 Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human
Tested Applications ELISA, IHC, IF
Dilution IHC:1:20-1:200, IF:1:50-1:200
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Solute carrier family 28 member 3 protein (1-102aa)
Antigen Species Human
Uniprot ID Q9HAS3
Target Name SLC28A3
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12548793/
Size 50ug

Image 1

IHC image of CSB-PA884504LA01HU diluted at 1:200 and staining in paraffin-embedded human Prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37℃ Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.

Image 2

Immunofluorescence staining of HepG2 cells with CSB-PA884504LA01HU at 1:200, counter-stained with DAPI.The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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