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Cusabio

SKU:CSB-PA857882LA01HU

AKAP9 Antibody

AKAP9 Antibody

Regular price $299.00 USD
Regular price Sale price $299.00 USD
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Size
Release Date 2022-04-01
Product Code CSB-PA857882LA01HU
Short Name AKAP9 Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human
Tested Applications ELISA, IHC
Dilution IHC:1:200-1:500
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human A-kinase anchor protein 9 protein (71-191aa)
Antigen Species Human
Uniprot ID Q99996
Target Name AKAP9
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12552795/
Size 100ug

Image 1

IHC image of CSB-PA857882LA01HU diluted at 1:250 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Secondary antibody only control: uses 1% BSA instead of primary antibody

Image 2

IHC image of CSB-PA857882LA01HU diluted at 1:250 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Secondary antibody only control: uses 1% BSA instead of primary antibody

Image 3

IHC image of CSB-PA857882LA01HU diluted at 1:250 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

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