Cusabio
SKU:CSB-PA823452LA01HU
TBC1D15 Antibody
TBC1D15 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA823452LA01HU |
| Short Name | TBC1D15 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $600/100ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC, IF |
| Dilution | WB:1:500-1:2000,IHC:1:50-1:200,IF:1:100-1:300 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | PBS, 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Antigen | Recombinant human TBC1 domain family member 15 protein |
| Antigen Species | Human |
| Uniprot ID | Q8TC07 |
| Target Name | TBC1D15 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12555174/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: A549 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), Hela whole cell lysate(30µg), 293T whole cell lysate(30µg), JK whole cell lysate(30µg), A431 whole cell lysate(30µg), SY5Y whole cell lysate(30µg),Mouse Brain tissue lysate(30µg),Mouse Liver tissue lysate(30µg)
All lanes: TBC1D15 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 80,78,79 kDa
Observed band size: 80 kDa
Exposure time: 30s
Image 2
IHC image of CSB-PA823452LA01HU diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 3
IHC image of CSB-PA823452LA01HU diluted at 1:50 and staining in paraffin-embedded humancolorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 4
Immunofluorescence staining of Hela cell with CSB-PA823452LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 5
Immunofluorescence staining of HepG2 cell with CSB-PA823452LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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