Cusabio
SKU:CSB-PA822255LA01HU
PRIMPOL Antibody
PRIMPOL Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA822255LA01HU |
| Short Name | PRIMPOL Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $600/100ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:1000-1:5000, IHC:1:50-1:200 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | PBS, 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Antigen | Recombinant human DNA-directed primase/polymerase protein (305-537aa) |
| Antigen Species | Human |
| Uniprot ID | Q96LW4 |
| Target Name | PRIMPOL |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12258568/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, JK whole cell lysate
All lanes: PRIMPOL antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
Image 2
IHC image of CSB-PA822255LA01HU diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 3
IHC image of CSB-PA822255LA01HU diluted at 1:50 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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