Cusabio
SKU:CSB-PA740922HA01HU
CLEC9A Antibody
CLEC9A Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA740922HA01HU |
| Short Name | CLEC9A Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $600/100ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IF |
| Dilution | WB:1:1000-1:5000, IF:1:50-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human C-type lectin domain family 9 member A protein (103-214aa) |
| Antigen Species | Human |
| Uniprot ID | Q6UXN8 |
| Target Name | CLEC9A |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12257861/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: MCF7 whole cell lysate, JK whole cell lysate, Raji whole cell lysate, THP-1 whole cell lysate, K562 whole cell lysate,U937 whole cell lysate,Mouse Kidney tissue lysate
All lanes: CLEC9A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 27 kDa
Observed band size: 35 kDa
Image 2
Immunofluorescence staining of Hela cells with CSB-PA740922HA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 3
Immunofluorescence staining of MCF-7 cells with CSB-PA740922HA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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