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Cusabio

SKU:CSB-PA618986LA01HU

SIX1 Antibody

SIX1 Antibody

Regular price $166.00 USD
Regular price Sale price $166.00 USD
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Size
Release Date 2022-04-01
Product Code CSB-PA618986LA01HU
Short Name SIX1 Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC
Dilution WB:1:500-1:5000, IHC:1:200-1:500
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Homeobox protein SIX1 protein (144-262aa)
Antigen Species Human
Uniprot ID Q15475
Target Name SIX1
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12552427/
Size 50ug

Image 1

Western Blot

Positive WB detected in: LO2 whole cell lysate, PC-3 whole cell lysate, NIH/3T3 whole cell lysate, Rat liver tissue

All lanes: SIX1 antibody at 2.7ug/ml

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

Image 2

IHC image of CSB-PA618986LA01HU diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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