Cusabio
SKU:CSB-PA618785LA01HU
RIPK1 Antibody
RIPK1 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA618785LA01HU |
| Short Name | RIPK1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Rat, Mouse |
| Tested Applications | ELISA, WB, IF |
| Dilution | WB:1:500-1:5000, IF:1:200-1:500 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Receptor-interacting serine/threonine-protein kinase 1 protein (328-458aa) |
| Antigen Species | Human |
| Uniprot ID | Q13546 |
| Target Name | RIPK1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12781312/ |
| Size | 100ug |
Image 1
Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, Rat liver tissue, Mouse heart tissue, Mouse kidney tissue
All lanes: RIPK1 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 76, 71 kDa
Observed band size: 76 kDa
Image 2
Immunofluorescence staining of HepG2 cells with CSB-PA618785LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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