Cusabio
SKU:CSB-PA613402LA01HU
OS9 Antibody
OS9 Antibody
Couldn't load pickup availability
| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA613402LA01HU |
| Short Name | OS9 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ul, $166/50ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:500-1:2000,IHC:1:50-1:200 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Protein OS-9 protein (232-413aa) |
| Antigen Species | Human |
| Uniprot ID | Q13438 |
| Target Name | OS9 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12552348/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: HepG2 whole cell lysate(30µg), 293 whole cell lysate(30µg), HL-60 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), U87 whole cell lysate(30µg), LO2 whole cell lysate(30µg),Mouse Liver tissue lysate(30µg)
All lanes: OS9 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 76,70,68,74,66.64.61 kDa
Observed band size: 70 kDa
Exposure time: 120s
Image 2
IHC image of CSB-PA613402LA01HU diluted at 1:50 and staining in paraffin-embedded human Lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Share
