Cusabio
SKU:CSB-PA11399A0Rb
ESR1 Antibody
ESR1 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA11399A0Rb |
| Short Name | ESR1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Rat |
| Tested Applications | ELISA, WB, IHC, ChIP |
| Dilution | WB:1:1000-1:5000, IHC:1:20-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Estrogen receptor protein (10-591aa) |
| Antigen Species | Human |
| Uniprot ID | P03372 |
| Target Name | ESR1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/1081334/ |
| Size | 100ug |
Image 1
Western blot
All lanes: ESR1 antibody at 7µg/ml
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: Rat brain tissue
Lane 4: Colo320 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 67, 54, 48, 36 kDa
Observed band size: 67 kDa
Image 2
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA11399A0Rb at dilution of 1:100
Image 3
Chromatin Immunoprecipitation MCF-7 (1.1*10<sup>6</sup>) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-ESR1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP11399HU) against the ESR1 promoter.
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