Cusabio
SKU:CSB-PA10749A0Rb
CEACAM1 Antibody
CEACAM1 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA10749A0Rb |
| Short Name | CEACAM1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IF |
| Dilution | WB:1:1000-1:5000, IF:1:200-1:500 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Carcinoembryonic antigen-related cell adhesion molecule 1 protein (35-428aa) |
| Antigen Species | Human |
| Uniprot ID | P13688 |
| Target Name | CEACAM1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11106924/ |
| Size | 50ug |
Image 1
Immunofluorescence staining of HepG2 cells with CSB-PA10749A0Rb at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 2
Western blot
All lanes: CEACAM1 antibody at 10µg/ml
Lane 1: HT29 whole cell lysate
Lane 2: HCT116 whole cell lysate
Lane 3: COLO205 whole cell lysate
Lane 4: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 58, 46, 36, 39, 51, 47, 28, 44, 52, 40 kDa
Observed band size: 51 kDa
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