• TRIM28 Antibody
  • TRIM28 Antibody
  • TRIM28 Antibody
  • TRIM28 Antibody
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Cusabio

CSB-PA05254A0Rb

TRIM28 Antibody

TRIM28 Antibody

$166.00 USD
$166.00 USD
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Size
Release Date 2022-04-01
Product Code CSB-PA05254A0Rb
Short Name TRIM28 Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IP
Dilution WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Transcription intermediary factor 1-beta protein (22-291aa)
Antigen Species Human
Uniprot ID Q13263
Target Name TRIM28
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/57891/
Size 50ug

Image 1

Western Blot

Positive WB detected in: PC-3 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate

All lanes: TRIM28 antibody at 1.5ug/ml

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 89, 80 kDa

Observed band size: 89 kDa

Image 2

Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA05254A0Rb at dilution of 1:100

Image 3

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA05254A0Rb at dilution of 1:100

Image 4

Immunoprecipitating TRIM28 in Hela whole cell lysate

Lane 1: Rabbit control IgG(1ug)instead of CSB-PA05254A0Rb in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)

Lane 2: CSB-PA05254A0Rb(6ug)+ Hela whole cell lysate(500ug)

Lane 3: Hela whole cell lysate (10ug)

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