Cusabio
SKU:CSB-PA05254A0Rb
TRIM28 Antibody
TRIM28 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA05254A0Rb |
| Short Name | TRIM28 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IP |
| Dilution | WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Transcription intermediary factor 1-beta protein (22-291aa) |
| Antigen Species | Human |
| Uniprot ID | Q13263 |
| Target Name | TRIM28 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/57891/ |
| Size | 100ug |
Image 1
Western Blot
Positive WB detected in: PC-3 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate
All lanes: TRIM28 antibody at 1.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 89, 80 kDa
Observed band size: 89 kDa
Image 2
Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA05254A0Rb at dilution of 1:100
Image 3
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA05254A0Rb at dilution of 1:100
Image 4
Immunoprecipitating TRIM28 in Hela whole cell lysate
Lane 1: Rabbit control IgG(1ug)instead of CSB-PA05254A0Rb in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA05254A0Rb(6ug)+ Hela whole cell lysate(500ug)
Lane 3: Hela whole cell lysate (10ug)
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