Cusabio
SKU:CSB-PA03987A0Rb
RELA Antibody
RELA Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA03987A0Rb |
| Short Name | RELA Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC, IF, ChIP |
| Dilution | WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Transcription factor p65 protein (1-210aa) |
| Antigen Species | Human |
| Uniprot ID | Q04206 |
| Target Name | RELA |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11106202/ |
| Size | 100ug |
Image 1
Western Blot
Positive WB detected in: 293 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: RELA antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 61, 59, 60 kDa
Observed band size: 61 kDa
Image 2
IHC image of CSB-PA03987A0Rb diluted at 1:600 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 3
IHC image of CSB-PA03987A0Rb diluted at 1:600 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 4
Immunofluorescence staining of PC-3 cells with CSB-PA03987A0Rb at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 5
Chromatin Immunoprecipitation Hela (1.2*10<sup>6</sup>) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-RELA or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP03987HU) against the IkBα promoter.
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