Cusabio
SKU:CSB-PA03604A0Rb
TRAP1 Antibody
TRAP1 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA03604A0Rb |
| Short Name | TRAP1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IF, IP |
| Dilution | WB:1:1000-1:5000, IF:1:50-1:200, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Heat shock protein 75 kDa, mitochondrial protein (60-308aa) |
| Antigen Species | Human |
| Uniprot ID | Q12931 |
| Target Name | TRAP1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/57887/ |
| Size | 50ug |
Image 1
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Mouse heart tissue
All lanes: TRAP1 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 81, 75 kDa
Observed band size: 81 kDa
Image 2
Immunofluorescent analysis of Hela cells using CSB-PA03604A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Image 3
Immunoprecipitating TRAP1 in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA03604A0Rb in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA03604A0Rb (8µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (10µg)
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