Cusabio
SKU:CSB-PA026183KA01HU
XBP1 Antibody
XBP1 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA026183KA01HU |
| Short Name | XBP1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $398/100ul |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IF |
| Dilution | WB:1:500-1:2000, IF:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Antigen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human XBP1. |
| Antigen Species | Human |
| Uniprot ID | P17861 |
| Target Name | XBP1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/158103/ |
| Size | 100ul |
Image 1
Western blot
All lanes: XBP1 antibody at 1:1000 dilution
Lane 1: 293T whole cell lysate
Lane 2: NCI-H460 whole cell lysate
Lane 3: Mouse eye tissue
Lane 4: Rat liver tissue
Lane 5: Rat eye tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29, 41 kDa
Observed band size: 41 kDa
Image 2
Immunofluorescent analysis of U2OS cells using CSB-PA026183KA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 3
Immunofluorescence staining of U2OS cells with CSB-PA026183KA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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