Cusabio
SKU:CSB-PA026138LA01HU
WNT5A Antibody
WNT5A Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA026138LA01HU |
| Short Name | WNT5A Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ul, $166/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:500-1:5000, IHC:1:20-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | Recombinant human Protein Wnt-5a protein (330-360aa) |
| Antigen Species | Human |
| Uniprot ID | P41221 |
| Target Name | WNT5A |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12552001/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: Hela whole cell lysate(30µg), MCF7 whole cell lysate(30µg), 293 whole cell lysate(30µg))
All lanes: WNT5A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 43, 41 kDa
Observed band size: 43 kDa
Exposure time: 120s
Image 2
IHC image of CSB-PA026138LA01HU diluted at 1:50 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 3
IHC image of CSB-PA026138LA01HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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