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Cusabio

SKU:CSB-PA025152LA01HU

TSPAN15 Antibody

TSPAN15 Antibody

Regular price $166.00 USD
Regular price Sale price $166.00 USD
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Size
Release Date 2022-04-01
Product Code CSB-PA025152LA01HU
Short Name TSPAN15 Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human
Tested Applications ELISA, WB, IF
Dilution WB:1:500-1:2000, IF:1:50-1:200
Purification Method Antigen affinity purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Tetraspanin-15 protein (115-235aa)
Antigen Species Human
Uniprot ID O95858
Target Name TSPAN15
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/11109440/
Size 50ug

Image 1

Immunofluorescence staining of A549 cell with CSB-PA025152LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Image 2

Western Blot

Positive WB detected in: MCF7 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, JK whole cell lysate, 293 whole cell lysate

All lanes: TSPAN15 antibody at 1:1000

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

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