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Cusabio
SKU:CSB-PA023446LA01MO
Tgfb1 Antibody
Tgfb1 Antibody
Regular price
$299.00 USD
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$299.00 USD
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA023446LA01MO |
| Short Name | Tgfb1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Mouse, Human |
| Tested Applications | ELISA, WB, IP |
| Dilution | WB:1:500-1:5000, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant mouse Transforming growth factor beta-1 protein (30-278aa) |
| Antigen Species | Mouse |
| Uniprot ID | P04202 |
| Target Name | Tgfb1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12549165/ |
| Size | 100ug |
Image 1
Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: Tgfb1 antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Image 2
Immunoprecipitating Tgfb1 in MCF-7 whole cell lysate
Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA023446LA01MO in MCF-7 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA023446LA01MO (8ug) + MCF-7 whole cell lysate (500ug)
Lane 3: MCF-7 whole cell lysate (10ug)
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