Cusabio
SKU:CSB-PA020071LA01HU
RORC Antibody
RORC Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA020071LA01HU |
| Short Name | RORC Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:1000-1:5000, IHC:1:20-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Nuclear receptor ROR-gamma protein (1-518aa) |
| Antigen Species | Human |
| Uniprot ID | P51449 |
| Target Name | RORC |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/1090217/ |
| Size | 100ug |
Image 1
Western Blot
Positive WB detected in: HepG2 whole cell lysate
All lanes: RORC antibody at 5.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Image 2
Western Blot
Positive WB detected in: HT29 whole cell lysate, MCF7 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate
All lanes: RORC antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59, 56 kDa
Observed band size: 59 kDa
Image 3
IHC image of CSB-PA020071LA01HU diluted at 1:1000 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 4
IHC image of CSB-PA020071LA01HU diluted at 1:1000 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 5
IHC image of CSB-PA020071LA01HU diluted at 1:1000 and staining in paraffin-embedded mouse liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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