Cusabio
SKU:CSB-PA019677LA01HU
RHD Antibody
RHD Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA019677LA01HU |
| Short Name | RHD Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IF |
| Dilution | WB:1:1000-1:3000, IF:1:20-1:100 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | PBS, 50% Glycerol, 0.5% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | Synthesized peptide derived from 161-210aa of Blood group Rh(D) polypeptide |
| Antigen Species | Human |
| Uniprot ID | Q02161 |
| Target Name | RHD |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12256403/ |
| Size | 50ug |
Image 1
Western Blot
Positive WB detected in: K562 whole cell lysate(20µg), Hela whole cell lysate(20µg), HepG2 whole cell lysate(20µg), LO2 whole cell lysate(20µg)
All lanes: RHD antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 46,35,54,44,42 kDa
Observed band size: 35 kDa
Exposure time: 60s
Image 2
Immunofluorescence staining of Hela cell with CSB-PA019677LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 3
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 4
Immunofluorescence staining of A549 cell with CSB-PA019677LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 5
Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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