Cusabio
SKU:CSB-PA019268LA01HU
RAD51 Antibody
RAD51 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA019268LA01HU |
| Short Name | RAD51 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC, IF, IP |
| Dilution | WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human DNA repair protein RAD51 homolog 1 protein (1-228aa) |
| Antigen Species | Human |
| Uniprot ID | Q06609 |
| Target Name | RAD51 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12256363/ |
| Size | 50ug |
Image 1
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate
All lanes: RAD51 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37, 27, 32 kDa
Observed band size: 37 kDa
Image 2
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA019268LA01HU at dilution of 1:100
Image 3
Immunofluorescent analysis of Hela cells using CSB-PA019268LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Image 4
Immunoprecipitating RAD51 in K562 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA019268LA01HU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA019268LA01HU (4µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (20µg)
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