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Cusabio

SKU:CSB-PA018670LA01HU

PRG2 Antibody

PRG2 Antibody

Regular price $299.00 USD
Regular price Sale price $299.00 USD
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Size
Release Date 2022-04-01
Product Code CSB-PA018670LA01HU
Short Name PRG2 Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ul, $166/50ul
Species Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC
Dilution WB:1:1000-1:5000, IHC:1:50-1:000
Purification Method Antigen affinity purification
Buffer Formulation PBS, 0.05% Sodium Azide, 40% Glycerol, pH 7.4.
Antigen Recombinant human Bone marrow proteoglycan protein (151-195aa)
Antigen Species Human
Uniprot ID P13727
Target Name PRG2
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12256312/
Size 100ul

Image 1

IHC image of CSB-PA018670LA01HU diluted at 1:66 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Secondary antibody only control: uses 1% BSA instead of primary antibody

Image 2

IHC image of CSB-PA018670LA01HU diluted at 1:66 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Secondary antibody only control: uses 1% BSA instead of primary antibody

Image 3

Western Blot

Positive WB detected in: Mouse spleen tissue lysate,Rat spleen tissue lysate

All lanes: PRG2 antibody at 1:1000

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 25, 28 kDa

Observed band size: 28 kDa

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