• PHB Antibody
  • PHB Antibody
  • PHB Antibody
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Cusabio

CSB-PA017885LA01HU

PHB Antibody

PHB Antibody

$299.00 USD
$299.00 USD
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Shipping calculated at checkout.
Size
Release Date 2022-04-01
Product Code CSB-PA017885LA01HU
Short Name PHB Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IP
Dilution WB:1:500-1:5000, IHC:1:200-1:500, IP:1:200-1:2000
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Prohibitin protein (1-272aa)
Antigen Species Human
Uniprot ID P35232
Target Name PHB
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/1026909/
Size 100ug

Image 1

IHC image of CSB-PA017885LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Image 2

Western Blot

Positive WB detected in: Raji whole cell lysate

All lanes: PHB antibody at 4.5µg/ml

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 30, 18 kDa

Observed band size: 30 kDa

Image 3

Immunoprecipitating PHB in HEK293 whole cell lysate

Lane 1: Rabbit control IgG (1µg) instead of CSB-PA017885LA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)

Lane 2: CSB-PA017885LA01HU (8µg) + HEK293 whole cell lysate (500µg)

Lane 3: HEK293 whole cell lysate (10µg)

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