Cusabio
SKU:CSB-PA017632LA01HU
PCOLCE2 Antibody
PCOLCE2 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA017632LA01HU |
| Short Name | PCOLCE2 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IF |
| Dilution | WB:1:500-1:2000, IF:1:20-1:100 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Procollagen C-endopeptidase enhancer 2 protein (24-415aa) |
| Antigen Species | Human |
| Uniprot ID | Q9UKZ9 |
| Target Name | PCOLCE2 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11110192/ |
| Size | 100ug |
Image 1
Western Blot
Positive WB detected in: A431 whole cell lysate(30µg), A549 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), 293 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), U87 whole cell lysate(30µg),A2780 whole cell lysate(30µg), Mouse Kidney tissue lysate(30µg)
All lanes: PCOLCE2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 120s
Image 2
Immunofluorescence staining of A549 cell with CSB-PA017632LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 3
Immunofluorescence staining of MCF-7 cell with CSB-PA017632LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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