Cusabio
SKU:CSB-PA017303LA01HU
OVOL1 Antibody
OVOL1 Antibody
Couldn't load pickup availability
| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA017303LA01HU |
| Short Name | OVOL1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $600/100ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:1000-1:3000, IHC:1:50-1:200 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | PBS, 0.02% Sodium Azide, 50% Glycerol, pH 7.4. |
| Antigen | Recombinant huamn Putative transcription factor Ovo-like 1 protein (9-267aa) |
| Antigen Species | Human |
| Uniprot ID | O14753 |
| Target Name | OVOL1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11111825/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: U251 whole cell lysate(20µg), MCF7 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), 293T whole cell lysate(20µg), A549 whole cell lysate(20µg),Mouse Brain tissue lysate(20µg)
All lanes: OVOL1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 30s
Image 2
IHC image of CSB-PA017303LA01HU diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 3
IHC image of CSB-PA017303LA01HU diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Share
