Cusabio
SKU:CSB-PA01555A0Rb
RPA2 Antibody
RPA2 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA01555A0Rb |
| Short Name | RPA2 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF, IP |
| Dilution | WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Replication protein A 32 kDa subunit protein (1-267aa) |
| Antigen Species | Human |
| Uniprot ID | P15927 |
| Target Name | RPA2 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/57443/ |
| Size | 100ug |
Image 1
Western Blot
Positive WB detected in: 293 whole cell lysate
All lanes: RPA2 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30, 31, 39 kDa
Observed band size: 30 kDa
Image 2
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA01555A0Rb at dilution of 1:100
Image 3
Immunofluorescent analysis of Hela cells using CSB-PA01555A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Image 4
Immunoprecipitating RPA2 in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA01555A0Rb in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA01555A0Rb (6µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
Image 5
Western Blot
Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, HT29 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
All lanes: RPA2 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30, 31, 39 kDa
Observed band size: 30 kDa
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