Cusabio
SKU:CSB-PA013471LA01HU
MAPK9 Antibody
MAPK9 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA013471LA01HU |
| Short Name | MAPK9 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human |
| Tested Applications | ELISA, IF, IP |
| Dilution | IF:1:200-1:500, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Mitogen-activated protein kinase 9 protein (1-424aa) |
| Antigen Species | Human |
| Uniprot ID | P45984 |
| Target Name | MAPK9 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11098007/ |
| Size | 50ug |
Image 1
Immunofluorescence staining of Hela cells with CSB-PA013471LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 2
Immunoprecipitating MAPK9 in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA013471LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA013471LA01HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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