MAG Antibody

Image 1

Immunofluorescence staining of MCF-7 cells with CSB-PA013322LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Image 2

Western Blot

Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate, Rat brain tissue, Mouse brain tissue

All lanes: MAG antibody at 5µg/ml

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 70, 64, 67 kDa

Observed band size: 70, 100 kDa

Image 3

IHC image of CSB-PA013322LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Image 4

IHC image of CSB-PA013322LA01HU diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.