Cusabio
SKU:CSB-PA012972EA01HU
LIPA Antibody
LIPA Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA012972EA01HU |
| Short Name | LIPA Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $600/100ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF |
| Dilution | WB:1:500-1:2000, IHC:1:20-1:200, IF:1:10-1:100 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | PBS, 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Antigen | Recombinant human Lysosomal acid lipase/cholesteryl ester hydrolase protein |
| Antigen Species | Human |
| Uniprot ID | P38571 |
| Target Name | LIPA |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/1028263/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: MCF7 whole cell lysate(30µg), 293T whole cell lysate(30µg), U251 whole cell lysate(30µg), A549 whole cell lysate(30µg), Hela whole cell lysate(30µg)
All lanes: LIPA antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size:46,40 kDa
Observed band size: 46 kDa
Exposure time: 10s
Image 2
IHC image of CSB-PA012972EA01HU diluted at 1:30 and staining in paraffin-embedded human Lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 3
IHC image of CSB-PA012972EA01HU diluted at 1:30 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 4
Immunofluorescence staining of HepG2 cell with CSB-PA012972EA01HU at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 5
Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 6
Immunofluorescence staining of PC-3 cell with CSB-PA012972EA01HU at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 7
Immunofluorescence staining of PC-3 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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