Cusabio
SKU:CSB-PA012769LA01HU
LATS1 Antibody
LATS1 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA012769LA01HU |
| Short Name | LATS1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human |
| Tested Applications | ELISA, IHC, IF, IP |
| Dilution | IHC:1:100-1:1200, IF:1:50-1:500, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Serine/threonine-protein kinase LATS1 protein (1-280aa) |
| Antigen Species | Human |
| Uniprot ID | O95835 |
| Target Name | LATS1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12255974/ |
| Size | 50ug |
Image 1
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA012769LA01HU at dilution of 1:100
Image 2
Immunofluorescent analysis of Hela cells using CSB-PA012769LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Image 3
Immunoprecipitating LATS1 in K562 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA012769LA01HU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA012769LA01HU (6µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)
Image 4
IHC image of CSB-PA012769LA01HU diluted at 1:1200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 5
IHC image of CSB-PA012769LA01HU diluted at 1:1200 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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