Cusabio
SKU:CSB-PA010902EA01HU
HTRA2 Antibody
HTRA2 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA010902EA01HU |
| Short Name | HTRA2 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IP |
| Dilution | WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Serine protease HTRA2, mitochondrial protein (334-458aa) |
| Antigen Species | Human |
| Uniprot ID | O43464 |
| Target Name | HTRA2 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12551611/ |
| Size | 50ug |
Image 1
IHC image of CSB-PA010902EA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 2
IHC image of CSB-PA010902EA01HU diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 3
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA010902EA01HU at dilution of 1:100
Image 4
Immunoprecipitating HTRA2 in MCF-7 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA010902EA01HU in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010902EA01HU (8µg) + MCF-7 whole cell lysate (500µg)
Lane 3: MCF-7 whole cell lysate (20µg)
Image 5
Western Blot
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, Raji whole cell lysate, HT29 whole cell lysate, HL60 whole cell lysate, K562 whole cell lysate
All lanes: HTRA2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 49, 47, 40 kDa
Observed band size: 39 kDa
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