Cusabio
SKU:CSB-PA010565LA01HU
HMGCR Antibody
HMGCR Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA010565LA01HU |
| Short Name | HMGCR Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:500-1:2000, IHC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | Synthesized peptide derived from human HMGCR |
| Antigen Species | Human |
| Uniprot ID | P04035 |
| Target Name | HMGCR |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/1042025/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: HepG2 whole cell
lysate(30µg), JK whole cell lysate(30µg), PC-3
whole cell lysate(30µg), A431 whole cell
lysate(30µg), THP-1 whole cell lysate(30µg),
K562 whole cell lysate(30µg),MCF7 whole cell
lysate(30µg),Mouse Brain tissue lysate(30µg)
All lanes: HMGCR antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 98, 93, 100 kDa
Observed band size: 98 kDa
Exposure time: 60s
Image 2
IHC image of CSB-PA010565LA01HU diluted at 1:150 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 3
IHC image of CSB-PA010565LA01HU diluted at 1:150 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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