Cusabio
SKU:CSB-PA010520LA01HU
HLTF Antibody
HLTF Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA010520LA01HU |
| Short Name | HLTF Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IP |
| Dilution | WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Helicase-like transcription factor protein (332-476aa) |
| Antigen Species | Human |
| Uniprot ID | Q14527 |
| Target Name | HLTF |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12781000/ |
| Size | 50ug |
Image 1
Western Blot
Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate
All lanes: HLTF antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 114, 100 kDa
Observed band size: 114 kDa
Image 2
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA010520LA01HU at dilution of 1:100
Image 3
Immunoprecipitating HLTF in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010520LA01HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA010520LA01HU (6ug) + Hela whole cell lysate (1mg)
Lane 3: Hela whole cell lysate (20ug)
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