Cusabio
SKU:CSB-PA010239LA01HU
HDAC3 Antibody
HDAC3 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA010239LA01HU |
| Short Name | HDAC3 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC, IF, ChIP |
| Dilution | WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Histone deacetylase 3 protein (1-428aa) |
| Antigen Species | Human |
| Uniprot ID | O15379 |
| Target Name | HDAC3 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11106203/ |
| Size | 100ug |
Image 1
Western blot
All lanes: HDAC3 antibody at 2µg/ml + Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49, 50 kDa
Observed band size: 49 kDa
Image 2
Western Blot
Positive WB detected in: Mouse heart tissue
All lanes: HDAC3 antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49, 50 kDa
Observed band size: 49 kDa
Image 3
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA010239LA01HU at dilution of 1:100
Image 4
Chromatin Immunoprecipitation Hela(1.2*10<sup>6</sup>)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-HDAC3 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP010239HU) against the P21 promoter.
Image 5
Immunofluorescent analysis of Hela cells using CSB-PA010239LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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