Cusabio
SKU:CSB-PA009706LA01HU
GPC4 Antibody
GPC4 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA009706LA01HU |
| Short Name | GPC4 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, IF |
| Dilution | WB:1:500-1:2000, IHC:1:20-1:200, IF:1:20-1:100 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Glypican-4 protein (19-210aa) |
| Antigen Species | Human |
| Uniprot ID | O75487 |
| Target Name | GPC4 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11107558/ |
| Size | 100ug |
Image 1
Western Blot
Positive WB detected in: MCF7 whole cell lysate(30µg), Mouse brain tissue lysate(30µg),Rat brain tissue lysate(30µg)
All lanes: GPC4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 55, 62 kDa
Observed band size: 55 kDa
Exposure time: 60s
Image 2
IHC image of CSB-PA009706LA01HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 3
IHC image of CSB-PA009706LA01HU diluted at 1:50 and staining in paraffin-embedded human kindey tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 4
Immunofluorescence staining of U251 cell with CSB-PA009706LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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