Cusabio
SKU:CSB-PA007340LA01HU
E2F1 Antibody
E2F1 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA007340LA01HU |
| Short Name | E2F1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC, IP |
| Dilution | WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Transcription factor E2F1 protein (133-362aa) |
| Antigen Species | Human |
| Uniprot ID | Q01094 |
| Target Name | E2F1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12255556/ |
| Size | 50ug |
Image 1
Western blot
All lanes: E2F1 antibody at 7 µg/ml + 3T3 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Image 2
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA007340LA01HU at dilution of 1:100
Image 3
Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA007340LA01HU at dilution of 1:100
Image 4
Immunoprecipitating E2F1 in 3T3 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA007340LA01HU in 3T3 whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA007340LA01HU (8µg) + 3T3 whole cell lysate (500µg)
Lane 3: 3T3 whole cell lysate (10µg)
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