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Cusabio

SKU:CSB-PA007111LA01HU

DOK5 Antibody

DOK5 Antibody

Regular price $299.00 USD
Regular price Sale price $299.00 USD
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Size
Release Date 2022-04-01
Product Code CSB-PA007111LA01HU
Short Name DOK5 Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, IF
Dilution WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Docking protein 5 protein (1-198aa)
Antigen Species Human
Uniprot ID Q9P104
Target Name DOK5
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/1028299/
Size 100ug

Image 1

Immunofluorescence staining of A549 cells with CSB-PA007111LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Image 2

Western blot

All lanes: DOK5 antibody at 2µg/ml + Mouse kidney tissue

Secondary

Goat polyclonal to rabbit IgG at 1/15000 dilution

Predicted band size: 36, 23 kDa

Observed band size: 36 kDa

Image 3

Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA007111LA01HU at dilution of 1:100

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