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Cusabio

SKU:CSB-PA006926LA01HU

DLAT Antibody

DLAT Antibody

Regular price $166.00 USD
Regular price Sale price $166.00 USD
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Size
Release Date 2022-04-01
Product Code CSB-PA006926LA01HU
Short Name DLAT Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human
Tested Applications ELISA, WB, IP
Dilution WB:1:500-1:5000, IP:1:200-1:2000
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial protein (26-217aa)
Antigen Species Human
Uniprot ID P10515
Target Name DLAT
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12555300/
Size 50ug

Image 1

Western Blot

Positive WB detected in: HepG2 whole cell lysate

All lanes: DLAT antibody at 4ug/ml

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

Image 2

Immunoprecipitating DLAT in HepG2 whole cell lysate

Lane 1: Rabbit control IgG instead of CSB-PA006926LA01HU in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)

Lane 2: CSB-PA006926LA01HU (6ug) + HepG2 whole cell lysate (1mg)

Lane 3: HepG2 whole cell lysate (20ug)

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