Cusabio
SKU:CSB-PA006621LA01HU
DDX3X Antibody
DDX3X Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA006621LA01HU |
| Short Name | DDX3X Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, IF, IP |
| Dilution | WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human ATP-dependent RNA helicase DDX3X protein (2-662aa) |
| Antigen Species | Human |
| Uniprot ID | O00571 |
| Target Name | DDX3X |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11098244/ |
| Size | 50ug |
Image 1
Western Blot
Positive WB detected in:293 whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, Mouse Brain tissue lysate, Rat Brain tissue lysate
All lanes:DDX3 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
Image 2
Immunofluorescence staining of Hela cell with CSB-PA006621LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 3
Immunohistochemistry analysis of human adrenal gland tissue using CSB-PA006621LA01HU at dilution of 1:100
Image 4
Immunoprecipitating DDX3X in Jurkat whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA006621LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA006621LA01HU (8µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (10µg)
Image 5
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 6
Immunofluorescence staining of HepG2 cell with CSB-PA006621LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 7
Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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