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Cusabio

SKU:CSB-PA00585A0Rb

TPMT Antibody

TPMT Antibody

Regular price $166.00 USD
Regular price Sale price $166.00 USD
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Size
Release Date 2022-04-01
Product Code CSB-PA00585A0Rb
Short Name TPMT Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IP
Dilution WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Thiopurine S-methyltransferase protein (4-244aa)
Antigen Species Human
Uniprot ID P51580
Target Name TPMT
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/57803/
Size 50ug

Image 1

Western blot

All lanes: TPMT antibody at 2µg/ml

Lane 1: EC109 whole cell lysate

Lane 2: 293T whole cell lysate

Secondary

Goat polyclonal to rabbit IgG at 1/15000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

Image 2

Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA00585A0Rb at dilution of 1:100

Image 3

Immunoprecipitating TPMT in K562 whole cell lysate

Lane 1: Rabbit control IgG instead of CSB-PA00585A0Rb in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)

Lane 2: CSB-PA00585A0Rb (8µg) + K562 whole cell lysate (500µg)

Lane 3: K562 whole cell lysate (10µg)

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