Cusabio
SKU:CSB-PA005755LA01HU
COL8A1 Antibody
COL8A1 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA005755LA01HU |
| Short Name | COL8A1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $600/100ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF |
| Dilution | WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | PBS, 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Antigen | Recombinant human Collagen alpha-1(VIII) chain protein (572-744aa) |
| Antigen Species | Human |
| Uniprot ID | P27658 |
| Target Name | COL8A1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11111053/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, PC-3 whole cell lysate
All lanes: COL8A1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
Image 2
Western Blot
Positive WB detected in: Mouse brain tissue lysate, Rat heart tissue lysate, Rat brain tissue lysate
All lanes: COL8A1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
Image 3
IHC image of CSB-PA005755LA01HU diluted at 1:67 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 4
Immunofluorescence staining of Hela cell with CSB-PA005755LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 5
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 6
Immunofluorescence staining of RAW264.7 cell with CSB-PA005755LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 7
Immunofluorescence staining of RAW264.7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 8
Immunofluorescence staining of NIH/3T3 cell with CSB-PA005755LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 9
Immunofluorescence staining of NIH/3T3 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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