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Cusabio
SKU:CSB-PA005571LA01HU
EGFR Antibody
EGFR Antibody
Regular price
$166.00 USD
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$166.00 USD
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA005571LA01HU |
| Short Name | EGFR Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IF |
| Dilution | WB:1:500-1:5000, IF:1:50-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Epidermal growth factor receptor protein (1067-1210aa) |
| Antigen Species | Human |
| Uniprot ID | P00533 |
| Target Name | EGFR |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12556261/ |
| Size | 50ug |
Image 1
Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: EGFR antibody at 2.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 135, 45, 78, 70 kDa
Observed band size: 135 kDa
Image 2
Immunofluorescence staining of HepG2 cells with CSB-PA005571LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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