Cusabio
SKU:CSB-PA005314LA01HU
CHAT Antibody
CHAT Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA005314LA01HU |
| Short Name | CHAT Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, IF |
| Dilution | WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | Synthesized peptide derived from human CHAT |
| Antigen Species | Human |
| Uniprot ID | P28329 |
| Target Name | CHAT |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12547991/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Mouse liver tissue
All lanes: CHAT antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 83, 75, 71 kDa
Observed band size: 75 kDa
Image 2
Western Blot
Positive WB detected in: Mouse Brain tissue lysate,Rat Brain tissue lysate
All lanes: CHAT antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47-83 kDa
Observed band size: 70 kDa
Image 3
IHC image of CSB-PA005314LA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 4
Immunofluorescent analysis of HepG2 cells using CSB-PA005314LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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