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Cusabio

SKU:CSB-PA004808LA01HU

CCNB2 Antibody

CCNB2 Antibody

Regular price $166.00 USD
Regular price Sale price $166.00 USD
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Size
Release Date 2022-04-01
Product Code CSB-PA004808LA01HU
Short Name CCNB2 Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human
Tested Applications ELISA, WB, IF
Dilution WB:1:500-1:5000, IF:1:50-1:200
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human G2/mitotic-specific cyclin-B2 protein (7-163aa)
Antigen Species Human
Uniprot ID O95067
Target Name CCNB2
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12780872/
Size 50ug

Image 1

Western Blot

Positive WB detected in: A549 whole cell lysate

All lanes: CCNB2 antibody at 2.5µg/ml

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

Image 2

Immunofluorescence staining of Hela cells with CSB-PA004808LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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