Cusabio
SKU:CSB-PA00367A0Rb
GPI Antibody
GPI Antibody
Couldn't load pickup availability
| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA00367A0Rb |
| Short Name | GPI Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC, IF, IP |
| Dilution | WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Glucose-6-phosphate isomerase protein (2-554aa) |
| Antigen Species | Human |
| Uniprot ID | P06744 |
| Target Name | GPI |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/1027231/ |
| Size | 50ug |
Image 1
Western Blot
Positive WB detected in: PC-3 whole cell lysate, Hela whole cell lysate, Mouse kidney tissue, Mouse brain tissue
All lanes: GPI antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64, 65 kDa
Observed band size: 64 kDa
Image 2
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00367A0Rb at dilution of 1:100
Image 3
Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA00367A0Rb at dilution of 1:100
Image 4
Immunofluorescent analysis of PC-3 cells using CSB-PA00367A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Image 5
Immunoprecipitating GPI in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA00367A0Rb in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA00367A0Rb (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
Share
