Cusabio
SKU:CSB-PA002344HA01HU
ATP5F1A Antibody
ATP5F1A Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA002344HA01HU |
| Short Name | ATP5F1A Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, IP |
| Dilution | WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human ATP synthase subunit alpha, mitochondrial protein (59-257aa) |
| Antigen Species | Human |
| Uniprot ID | P25705 |
| Target Name | ATP5F1A |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12548922/ |
| Size | 100ug |
Image 1
Western Blot
Positive WB detected in: K562 whole cell lysate, Mouse heart tissue
All lanes: ATP5F1A antibody at 2.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 55, 58 kDa
Observed band size: 60 kDa
Image 2
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA002344HA01HU at dilution of 1:100
Image 3
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA002344HA01HU at dilution of 1:100
Image 4
Immunoprecipitating ATP5A1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of (1?g) instead of CSB-PA002344HA01HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA002344HA01HU (4?g) + Hela whole cell lysate (500?g)
Lane 3: Hela whole cell lysate (20?g)
Image 5
Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Mouse brain tissue, Rat brain tissue
All lanes: ATP5A1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 55, 58 kDa
Observed band size: 60 kDa
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