Cusabio
SKU:CSB-PA001836YA01MO
Anxa1 Antibody
Anxa1 Antibody
Couldn't load pickup availability
| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA001836YA01MO |
| Short Name | Anxa1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Mouse, Human |
| Tested Applications | ELISA, WB, IP |
| Dilution | WB:1:1000-1:5000, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant mouse Annexin A1 protein (2-346aa) |
| Antigen Species | Mouse |
| Uniprot ID | P10107 |
| Target Name | Anxa1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/172625/ |
| Size | 100ug |
Image 1
Western blot
All lanes: Anxa1 antibody at 2µg/ml
Lane 1: PC-3 whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: K562 whole cell lysate
Lane 4: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Image 2
Western Blot
Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate
All lanes: Anxa1 antibody at 2.9µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Image 3
Immunoprecipitating Anxa1 in NIH/3T3 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA001836YA01MO in NIH/3T3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA001836YA01MO (8µg) + NIH/3T3 whole cell lysate (500µg)
Lane 3: NIH/3T3 whole cell lysate (20µg)
Share
