Cusabio
SKU:CSB-PA001453LA01HU
AGPAT5 Antibody
AGPAT5 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA001453LA01HU |
| Short Name | AGPAT5 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $297/100ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:500-1:2000, IHC:1:20-1:200 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | PBS, 150mM NaCl, 0.02% Sodium Azide, 50% Glycerol, pH 7.4. |
| Antigen | Synthesized peptide derived from internal of human AGPAT5 |
| Antigen Species | Human |
| Uniprot ID | Q9NUQ2 |
| Target Name | AGPAT5 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12555256/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: B16F10 whole cell lysate(30µg), A2780 whole cell lysate(30µg), Hela whole cell lysate(30µg), HL-60 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), K562 whole cell lysate(20µg),SY5Y whole cell lysate(30µg),293 whole cell lysate(30µg),Mouse Brain tissue lysate(20µg)
All lanes: AGPAT5 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 60s
Image 2
IHC image of CSB-PA001453LA01HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 3
IHC image of CSB-PA001453LA01HU diluted at 1:100 and staining in paraffin-embedded human Liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 4
IHC image of CSB-PA001453LA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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