Cusabio
SKU:CSB-MA554946
HSP90B1 Monoclonal Antibody
HSP90B1 Monoclonal Antibody
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| Release Date | 2025-12-04 |
|---|---|
| Product Code | CSB-MA554946 |
| Short Name | HSP90B1 Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, FC, ICC |
| Dilution | WB:1:400-1:2000, FC : 1:200-1:2000, ICC:1:100-1:1000 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 0.02% Sodium Azide, 50% Glycerol, pH 7.4. |
| Antigen | Recombinant Human HSP90B1 protein |
| Antigen Species | Human |
| Uniprot ID | P14625 |
| Target Name | HSP90B1 |
| Clonality | Monoclonal |
| Clone ID | 3C3 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12947872/ |
| Size | 100ul |
Image 1
Flow cytometric analysis of HSP90B1 expression in HAP-1 cells using HSP90B1 antibody. Green, isotype control; red, HSP90B1.
Image 2
Immunocytochemical staining of HAP-1 cells with HSP90B1 antibody. Nuclei were stained blue with DAPI; HSP90B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Image 3
Western blotting analysis using HSP90B1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSP90B1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively.
Image 4
Western blotting analysis using HSP90B1 antibody. HSP90B1 expression in wild-type (WT) and HSP90B1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with HSP90B1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively.
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