CLIA Kit for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

13.7-10,000pg/mL

Application

Chemiluminescent immunoassay for Antigen Detection.

Organism species

Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.9pg/mL

Sample type

Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

2h, 40min

Method

Double-antibody Sandwich

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) level in the sample or standard.;

Alternative Names

Item Name

Wingless Type MMTV Integration Site Family, Member 5A

Research Area

Signal transduction;

Reference